GENOMIC SEQUENCING
Contacts:
Ken Stuart: kstuart@u.washington.edu
Peter Myler: mylerpj@sbri.org
The rate of progress in the field of Leishmania genome mapping over the last eighteen months had been sufficiently great that the next stage of genome dissection, the determination of the primary DNA sequence, became a realistic objective. Such an
approach is known to provide a rich source of both structural and biological information, especially for organisms with small genomes.
A similar scheme has been adopted for Leishmania, applying the various complementary approaches available within the LGN: the use of chromosome-specific markers to identify cosmid clones, and hence contigs, that constitute the "minimal tile set",
for a given chromosome. Undertaken in Ken Stuart's laboratory, a pilot sequencing project of chromosome 1 is nearing completion.
Annotated sequence data from the first Leishmania cosmids to be sequenced:
have been lodged in GenBank and in the central LGN database LeishDB.
CONNECT ME TO:
the S.B.R.I. LeishGen Web Page
EST BLASTN, BLASTX data or FTP the late st FASTA results
a table of ALL Leishmania sequences (with ENTREZ links)
a table of Leishmania EST sequences (with ENTREZ links)
ENTREZ to RETRIEVE all Leishmania sequences
NCBI's ENTREZ interface
EBI's Sequence Analysis interface
INDEX of all Leishmania Genome Network WWW pages
LGN HOME PAGE
Last modified: 13 JAN 1997