PHYSICAL MAPPING OF THE Leishmania major FRIEDLIN GENOME

Contact:

Al Ivens, Department of Biochemistry, Imperial College, Exhibition Rd., London SW7 2AZ, UK. Email: a.ivens@ic.ac.uk
Organisms with small genomes, most notably C. elegans, have been studied extensively using clone-based methods (cosmids), leading onto large-scale sequencing projects. The cosmid-based approach has been applied to the study of the Leishmania ma jor Freidlin genome, using the technique of fingerprinting. A cosmid library of 9216 clones (9-fold genome coverage), constructed in the shuttle vector cLHYG (Ryan et al., 1993: Gene 131:145-150), acts as a WHO L.G.N. refe rence resource. The otherwise anonymous clones have been assembled into overlapping "contigs" by virtue of the pattern of fragments generated by simple restriction digestion (HinfI) and end-filling labelling reactions. Autoradiographs of electroph oretically-separated products were computer analysed by the Image v2.1 software package to generate a clone database; contigs within this database were assembled using the contasp and FPC software packages, which aligned the clones on the basis of their fingerprints. The cosmid library described above has been gridded on membranes for hybridsation analyses with DNA probes.

Give me the:

  • latest fingerprint and contig statistics

  • current activities and data

  • summary of LGN hybridisation data

  • INDEX of all Leishmania Genome Network WWW pages

  • LGN HOME PAGE

    • Last modified: 13 JAN 1997