SCANNED PICTURES OF PFG-SEPARATED Leishmania CHROMOSOMES

The pictures for PFG-separated Leishmania chromosomes come from two sources:
  • Patrick Bastien and colleagues (Montpellier, France) [Wincker et al., 1996: Nuc. Ac. Res.24:1688-1694] Email : gpp@sc.univ-montp1.fr
  • Jennie Blackwell and colleagues (Cambridge,UK) Email: jmb37@cus.cam.ac.uk

    PATRICK BASTIEN

    Equipment: see Wincker et al. 1996 for details
    Markers: S. cerevisiae AB1380, Hansenula wingei (BioRad)
    Agarose: ???
    1xTBE: 89mM Tris-HCl/89mM boric acid/2mM EDTA (pH7.4)

    SEPARATIONS

  • See Wincker et al., 1996 for details Email : gpp@sc.univ-montp1.fr
    1) 1.0-3.0 Mb??? VIEW or FTP the picture (149k file)
    2) 0.9-2.0 Mb???VIEW or FTP the picture (244k file)
    3) 0.2-0.9 Mb???VIEW or FTP the picture (283k file)

    JENNIE BLACKWELL

    Equipment: Biorad CHEF-DR2.
    Markers: S. cerevisiae YPH80; New England Biolabs #345
    (Sizes (kb): 1900, 1640, 1120, 1100, 945, 915, 815, 785, 745, 680, 610, 555, 450, 375, 295, 225).

    Agarose: Pharmacia #17-0554-03.
    10xTBE: 108g Tris base/55g boric acid/4mL 0.5M EDTA (pH8.0)/water to 1L.

    SEPARATIONS

    (all use: 1x TBE, 12C run temp., 1% agarose, 85 Volts).
    1) 0.7-3.0 Mb850-450 seconds ramp for 144 h. VIEW or FTP the picture (80k file)
    2) 0.1-1.0 Mb175 seconds for 120 h. VIEW or FTP the picture (82k file)
    3) 0.1-1.6 Mb500-200 seconds ramp for 96 h. VIEW or FTP the picture (82k file)
    4) 0.1-2.0 Mb400-200 seconds ramp for 144 h. VIEW or FTP the picture (100k file)< /TH>

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    • Last modified: 13 JAN 1997