DESCRIPTION
Using DNA synthesizer, oligonucleotides are produced from phosphoramidites following the programmed sequence on a solid support. The oligonucleotides are purified by TLC, FPLC, and HPLC and submitted to quality control by labelling and electrophoresis.
MAIN ADVANTAGES
Automated production of oligonucleotides is crucial for Genetic Engineering and the development of diagnostic methods with probes and PCR.
MAIN APPLICATIONS
Diagnostic probes, vector construction, screening of genomic libraries, typing of organisms, etc.
STAGE OF DEVELOPMENT
Routine production.
PERSONS IN CHARGE
Philip Suffys and Wim Maurits Degrave
UNIT/DEPARTMENT/DIVISION
Instituto Oswaldo Cruz/Biochemistry and Molecular Biology Department