in ~cmungall/data you will find a directory called new-ests/ in this directory there are various EST sequences in individual fasta files. some of these are 3 prime some are 5 rpime using only unix pipes, the unix grep command and the two programs in ~cmungall/data try and make a combined fasta file containing only 5 prime sequences you should be able to do this in one step. hint: the program fasta2flat.pl can be used to convert fasta to a flattened format containing one line per sequence, and flat2fasta.pl can be used to convert back to fasta