LOCUS       MP34KDA      1832 bp    DNA             BCT       06-OCT-1993
DEFINITION  M.paratuberculosis gene for 34 kDa protein.
ACCESSION   X68102
KEYWORDS    .
SOURCE      Mycobacterium paratuberculosis.
  ORGANISM  Mycobacterium paratuberculosis
            Eubacteria; Firmicutes; Actinomycetes; Mycobacteria;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Gilot,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-1992) to the EMBL/GenBank/DDBJ databases. P.
            Gilot, International Inst. of Cellular and Molecular Pathology,
            GEMO, UCL 7449, Ave. Hippocrate, 75, 1200 Bruxelles, BELGIUM
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Gilot,P., De Kesel,M., Machtelinckx,L., Coene,M. and Cocito,C.
  TITLE     Isolation and sequencing of the gene coding for an antigenic
            34-kilodalton protein of Mycobacterium paratuberculosis
  JOURNAL   J. Bacteriol. 175 (15), 4930-4935 (1993)
  MEDLINE   93328703
COMMENT     NCBI gi: 44475
FEATURES             Location/Qualifiers
     source          1. .1832
                     /organism="Mycobacterium paratuberculosis"
                     /strain="ATCC 19698"
                     /clone_lib="M.paratuberculosis DNA in lambda gt11"
                     /sub_clone="in pBluescript Sk+"
     -35_signal      686. .691
     -10_signal      712. .717
     RBS             732. .737
     CDS             742. .1638
                     /note="NCBI gi: 44476"
                     /codon_start=1
                     /product="34 kDa protein"
                     /translation="MTYSPGSPGYPPAQSGGTYAGATPSFAKDDDGKSKLPLYLNIAV
                     VALGFAAYLLNFGPTFTIGADLGPGIGGRAGDAGTAVVVALLAALLAGLGLLPKAKSY
                     VGVVAVVAVLAALLAITETINLPAGFAIGWAMWPLVACVVLQAIAAVVVVLLDAGVIT
                     APAPRPKYDPYAQYGQYGQYGQYGQQPYYGQPGGQPGGQPGGQQHSPQGYGSQYGGYG
                     QGGAPTGGFGAQPSPQSGPQQSAQQQGPSTPPTGFPSFSPPPNVGGGSDSGSATANYS
                     EQAGGQQSYGQEPSSPSGPTPA"
BASE COUNT      275 a    640 c    635 g    282 t
ORIGIN

MP34KDA  Length: 1832  October  2, 1995 15:40  Type: N  Check: 1694  ..

       1  GGGCCCGAAC TTGACGAACT CGCCGTCGTA GCTGGCTTCC TCGTCGGTCC 

      51  ACAGCGCCCG CATCGCTTCC AGGTATTCGC GCAGCATGGT GCGGCGCCGG 

     101  CCCGCCGGCA CGCCGTGGTC GGCGAGTTCG TCGGTGTTCC AGCCGAACCC 

     151  GACGCCGAGG CTGACCCGGC CGCCGGACAG ATGGTCAAGG GTGGCAATAC 

     201  TTTTCGCCAG CGTGATCGGG TCGTGTTCGA CCGGCAGGGC CACCGCGGTG 

     251  GACAGCCGCA CCCGCGAGGT GACGGCACAG GCCGCGCCCA GACTGACCCA 

     301  CGGGTCCAGG GTGCGCATGT AGCGGTCGTC GGGCAGCGAC GCGTCGCCGG 

     351  TGGTCGGGTG CGCGGCCTCC CGCTTGATCG GGATATGCGT GTGTTCCGGC 

     401  ACGTAGAAGG TCGCAAACCC GTGGTCGTCG GCAAGCTTCG CGGCCGCAGC 

     451  CGGAGAGATG CCACGGTCGC TGGTGAAAAG CACAAGCCCG TAATCCATGC 

     501  AGTGAATTAG AACGTGTTCT ACCTCTGCGG GGCAAGCTGT CGTGATACGG 

     551  ACCGTCTCGC CGCGCGGTCG TCTGCGAAGC CCGCGGGCAA GCCAATGGCG 

     601  ACGGCACCGG CCGTCGCACG TGCGCTAGCG TGGGTGATCG ACCGTGTCGC 

     651  TCGCGCAGTG ACGCGCCTGC AAGCACCGCG TCGCATCGCA ACCGTGGCGC 

     701  CCGCTCGGCA CTAAAAGGCA GTGGAAGCAA CAGGAGGAGC CATGACCTAC 

     751  TCTCCCGGCA GCCCCGGATA TCCACCGGCG CAGTCTGGCG GCACCTATGC 

     801  AGGCGCCACA CCATCTTTCG CCAAAGACGA CGACGGCAAG AGCAAACTCC 

     851  CGCTCTACCT CAACATCGCC GTGGTCGCCC TGGGTTTCGC GGCCTACCTG 

     901  CTGAATTTCG GCCCCACCTT CACCATCGGC GCCGACCTCG GCCCGGGTAT 

     951  CGGCGGCCGC GCGGGTGACG CCGGCACCGC CGTCGTGGTG GCGCTGCTGG 

    1001  CCGCGCTGCT CGCCGGGCTG GGCCTGCTGC CCAAGGCCAA GAGTTATGTG 

    1051  GGCGTGGTCG CGGTCGTCGC GGTGCTCGCC GCGCTGCTGG CCATCACCGA 

    1101  GACGATCAAC CTGCCCGCCG GTTTCGCGAT CGGCTGGGCG ATGTGGCCGC 

    1151  TGGTGGCGTG CGTGGTGCTG CAGGCGATCG CCGCGGTGGT CGTGGTCCTG 

    1201  CTGGACGCCG GGGTGATCAC GGCGCCGGCG CCGCGGCCCA AGTACGACCC 

    1251  CTACGCGCAG TACGGCCAAT ACGGGCAATA CGGCCAGTAC GGGCAACAGC 

    1301  CCTACTACGG TCAGCCGGGC GGTCAGCCCG GGGGCCAGCC GGGTGGTCAG 

    1351  CAGCATTCGC CGCAGGGCTA CGGGTCGCAG TACGGCGGTT ACGGCCAGGG 

    1401  CGGCGCTCCG ACCGGCGGTT TCGGTGCCCA GCCGTCGCCG CAGTCCGGCC 

    1451  CGCAACAGTC CGCGCAGCAG CAGGGCCCGT CCACACCGCC CACCGGCTTC 

    1501  CCCAGCTTCA GCCCGCCGCC CAACGTCGGC GGGGGATCGG ACTCCGGTTC 

    1551  GGCGACCGCC AATTACTCCG AGCAGGCCGG TGGCCAGCAG TCCTACGGCC 

    1601  AGGAGCCTTC TTCACCGTCT GGGCCGACGC CCGCCTAACG TGCCCTGTCG 

    1651  CGCCTAGTCG GGAACGTGCC CCAGAGTGAC ACGGGTGGAG GACAACCGGG 

    1701  CAGCGGGCGC TCGCCAGGCG CGTGACCTCG TCAGGGTCGC GTTCGCCCCG 

    1751  GCGGTGGTGG CACTGGTCAT CATCGCCGCG GTCACGCTGA TCCAGTTGTT 

    1801  GATCGCCAAC AGCGACATGA CCGGCGCGTT GG