LOCUS       MPIS900       716 bp    DNA             BCT       26-JUL-1993
DEFINITION  M.paratuberculosis IS900 element DNA for ORF2.
ACCESSION   X69575
KEYWORDS    insertion element IS900.
SOURCE      Mycobacterium paratuberculosis.
  ORGANISM  Mycobacterium paratuberculosis
            Eubacteria; Firmicutes; Actinomycetes; Mycobacteria;
            Mycobacteriaceae; Mycobacterium.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Murray,A., Winter,N., Lagranderie,M., Hill,D.F., Rauzier,J.,
            Timm,J., Leclerc,C., Moriarty,K.M., Gheorghiu,M. and Gicquel,B.
  TITLE     Expression of Escherichia coli beta-galactosidase in Mycobacterium
            bovis BCG using an expression system isolated from Mycobacterium
            paratuberculosis which induced humoral and cellular immune
            responses
  JOURNAL   Mol. Microbiol. 6 (22), 3331-3342 (1992)
  MEDLINE   93133113
COMMENT     ORF2 is located in IS900 on the complementary strand to the gene
            coding for transposase.

            NCBI gi: 395189
FEATURES             Location/Qualifiers
     source          1. .716
                     /organism="Mycobacterium paratuberculosis"
                     /clone_lib="lambda gt11 genomic library"
                     /clone="pAM3"
                     /insertion_seq="IS900"
     -35_signal      83. .88
     -10_signal      106. .111
     prim_transcript 118. .>716
     RBS             149. .154
     CDS             160. .>716
                     /note="ORF2;  NCBI gi: 395190"
                     /codon_start=1
                     /translation="MTLSSRRGSGCGVVDSVVAQHGPQDVEAAAGQGEDGLGVAFSFG
                     AFSVVVGARGGVGADADQGRQVAGAQQAPVVASGAFEVSADAAGISWYRRQAGDAGEA
                     VGGGECGHVPAGGGEELSAQDDAESGHAQDDFGVAVAAKSLLDHRVGVADFGVEGHHL
                     LGQAGHHSGRQLLAGHDAVLGVSGL"
BASE COUNT      112 a    203 c    278 g    123 t
ORIGIN

 MPIS900  Length: 716  October  2, 1995 15:40  Type: N  Check: 2542  ..

       1  GATCCCGTGA CAAGGCCGAA GAGCCCGCGA CCGTGCGGTC GTCGACGACC 

      51  GAGTGTGAGC AGACCCCCTG GTGAAGGGTG AATCGACAGG TACACACAGC 

     101  CGCCATACAC TTCGCTTCAT GCCCTTACGG GGGGCGGCCA ACCCAGAAGG 

     151  AGATTCTCAA TGACGTTGTC AAGCCGCCGC GGTAGTGGTT GCGGGGTGGT 

     201  AGACAGCGTG GTCGCGCAGC ATGGCCCACA GGACGTTGAG GCGGCGGCGG 

     251  GCCAGGGCGA GGACGGCTTG GGTGTGGCGT TTTCCTTCGG TGCGTTTTCG 

     301  GTCGTAGTAG GTGCGCGAGG AGGGGTCGGT GCGGATGCTG ACCAAGGCCG 

     351  ACAGGTAGCA GGCGCGCAGC AGGCGCCGGT CGTAGCGTCG GGGGCGTTTG 

     401  AGGTTTCCGC TGATGCGGCC GGAATCTCGT GGTACCGGCG CCAGGCCGGC 

     451  GACGCCGGCG AGGCGGTCGG CGGAGGCGAA TGCGGCCATG TCCCCGCCGG 

     501  TGGCGGCGAG GAACTCAGCG CCCAGGATGA CGCCGAATCC GGGCATGCTC 

     551  AGGATGATTT CGGCGTGGCG GTGGCGGCGA AATCGCTCCT CGATCATCGC 

     601  GTCGGTGTCG CCGATTTCGG TGTCGAGGGC CATCACCTCC TTGGCCAGGC 

     651  GGGCCACCAC AGTGGCCGCC AGTTGTTGGC CGGGCACGAT GCTGTGTTGG 

     701  GCGTTAGCGG CCTGCA