The Trypanosoma brucei Project
Secretary to the project
Karyotype mapping
(Tait, Glasgow, Melville, Cambridge)
All the smaller smaller chromosomes have been carefully mapped by a combination
of genetic and hybridisation mapping.
Physical map
(Melville, Cambridge)
Many large contigs of BAC gDNA clones have been constructed by sts mapping
and walking strategies.
The large repetitive sequence and transposon content makes full mapping
of chromosomes difficult, but the central gene-rich regions should be amenable
to analysis in the first instance.
Physical mapping library and other genomic libraries are available to the
research community
Mapping of probes to library clones and karyotype is routine
EST sequencing
(Donelson, USA and Majiwa, Kenya)
2,015 ESTs have been deposited in the public db and another similar number
are to be submitted soon.
Genome Sequencing
(Donelson, USA)
A small pilot project suggests that this is both feasibile and useful: indeed
it may be an efficient route to gene discovery like the EST approach.
Genetics
(Tait, Glasgow)
Strains and molecular tools are now in hand to allow the addressing of biological
questions such as the genetics of drug resistance and host infectivity.
08/10/1996