CURRENT ACTIVITIES OF THE LONDON LABORATORY

Contact:

Al Ivens, Department of Biochemistry, Imperial College, Exhibition Rd., London SW7 2AZ, UK. Email: a.ivens@ic.ac.uk

Complementary methods of refinement, particularly the incorporation of probe hybridisation data, greatly augment the usefulness of the contig map framework. This is especially true if the chromosomal map location of the probe of interest is known. Hybri disation of mapped EST markers [Wincker et al., 1996: Nuc. Ac. Res. 24:1688-1694] to gridded arrays of the cLHYG cosmid library have assigned both biological tags and chromosomal map locations to the contigs identified. Data from ot her markers (ESTs, mapped genes and anonymous DNA probes) are continually adding more information to the "first generation" contig map.

Activites in the London laboratory currently include the joining of contigs by hybridisation of 497 single-copy contig "end-probes", assignment of contigs to chromosomes, the hybridisation and PFG mapping of EST probes, and the construction of "minimum ti le" sets of clones for genome sequence analysis.

Using high-density colony arrays of the cLHYG Leishmania major Friedlin cosmid library and/or PFG separated Friedlin chromosomes, the London laboratory has mapped:


74Genes and/or ESTs
121Anonymous DNA markers
86Contig "end-probes"
7Chromosomal probes
290TOTAL

  • Summary of LGN hybridisation data

  • INDEX of all Leishmania Genome Network WWW pages

  • LGN HOME PAGE

    • Last modified: 29 JAN 1997