EST SEQUENCING

Contacts:
  • Jennie Blackwell Email: jmb37@cus.cam.ac.uk
  • Horacio Schneider Email: horacio@cuxiu.cbio.ufpa.br and Ira Sampaio Email: ira@cuxiu.cbio.ufpa.br
  • Angela Cruz Email: akcruz@fmrp.usp.br
    The presence of the spliced leader RNA at the 5' end of all Leishmania transcripts has facilitated the construction of cDNA libraries containing high proportions of full-length molecules; libraries constructed from different developmental stage s of L. major have been used for sequencing. The strategy of random partial sequencing of cDNA clones to generate ESTs is not only a rapid method of gene identification, but provides a source of hybridisation markers that aid the construction of p hysical maps necessary for genomic sequencing.
    To date, 5' DNA sequences from a total of 527 Leishmania major ESTs have been submitted to dbEST and lodged in the LGN database LeishDB. The fi rst 268 sequences were submitted to the database by the Cambridge laboratory [Levick et al., 1996: Mol. Biochem. Parasitol. 76: 345-348], while virtually all other sequences have come from one of the Brazilian LGN laboratories. The LGN-agreed target is to generate a minimum of 1500 new sequences for Leishmania. ESTs are deposited in dbEST as they are generated, enabling rapid and easy access by the international research community.
    Approximately 30% of the new sequences have been assigned a putative identity based on significant similarity scores obtained by the basic local alignment search tool (BLAST). Around 10% show identity to ribosomal protein sequences, the remainder to a ra nge of genes involved in diverse cellular functions. The remaining 20% or so are NOVEL.
    Sequencing from log and metacyclic promastigote (Belem, Brazil), and amastigote (Cambridge, UK) cDNA libraries is continuing.

    TAKE ME TO:

  • a table of Leishmania EST sequences (with ENTREZ links)

  • EST BLASTN, BLASTX data or FTP the late st FASTA results

  • a summary of EST hybridisations to cosmid colony arrays or PFG-separated chromosomes

  • the current status of mapped EST markers on Leishmania major Friedlin chromosomes

  • scanned pictures of PFG-separated Leishmania chromosomes

  • a black and white idiogram of Leishmania chromosomes

  • a colour idiogram of Leishmania chromosomes

  • a table of ALL Leishmania sequences (with ENTREZ links)

  • ENTREZ to RETRIEVE all Leishmania sequences

  • INDEX of all Leishmania Genome Network WWW pages

  • LGN HOME PAGE

    • Last modified: 29 JAN 1997